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Background and Objectives:
There were over 110,000 acres of
forage seed produced in Manitoba in 2000. Registered options available
to both forage and forage seed growers remain limited causing a high
rate of unregistered pesticide use in both industries. To obtain a User
Requested Minor Use Label Expansion (URMULE), the Pest Management
Regulatory Agency requires at least 2-3 years of research that includes
crop tolerance and impacts on seed yield. Weed control issues have been
identified as the number one limitation to increasing seed yield and
quality in forage and grass seed crops across Manitoba. Development of
effective and economical weed control programs is necessary for the
industry to continue growing and maintain stability.
The Manitoba Forage Seed
Association conducted a broad-spectrum herbicide tolerance screening
trial in 2000. Results from this trial were used to determine products
to pursue for registration. Trials in 2001 and 2002 were coordinated
with government specialists from British Columbia, Alberta, and
Saskatchewan. Both seed trade and producers from western Canada were
consulted and provided valuable input. Trials in 2001 focused on
seedling crops while trials in 2002 targeted established crops.
Herbicides tested have a determined weed spectrum and a major
registration. Treatments included products to address both grassy and
broad leaf weed problems such as cleavers, wild oats, green foxtail
barnyard grass, Canada thistle, perennial sow-thistle, dandelion, as
well as others in forage seed crops.
Seedling Trials:
Procedure and Project
Activities
In 2001, large blocks of forage
seed crops were solid seeded in Arborg and St. Norbert, Manitoba to
conduct herbicide trials. Species seeded included alfalfa (Medicago
sativa), perennial ryegrass (Lolium perenne), timothy (Phleum
pratense), tall fescue (Festuca arundinacea) and annual
ryegrass (Lolium multiflorum). All species were successfully
established at Arborg while only perennial and annual ryegrasses were
successfully established at St. Norbert. Seedling grasses were sprayed
at the 2-4 leaf stage and seedling alfalfa was sprayed at the 3-4
trifoliate stage. Crop tolerance ratings, using the scale in Figure 1,
were taken approximately 3, 14 and 28 days after treatment. Plots were
2m x 6m and were sprayed with a bicycle sprayer. A 10g/acre (45L/acre)
water volume was used to spray all treatments. An area of 3m2
from each plot was clipped, bagged, dried and threshed to obtain seed
yields. The annual ryegrass plot in Arborg was not harvested for seed
yield due to a flooding period, which affected fertility and seed
production. As well, the timothy plot in Arborg was not taken to seed
yield to due poor establishment.
Figure 1. 0-100% Crop Tolerance
Ratings
|
100 |
Complete Tolerance |
|
90-80 |
Possible Effect |
|
70 |
Slight Effect |
|
60 |
Definite Effect |
|
50-10 |
Severe Effect |
|
0 |
Complete Kill |
< 70% crop tolerance to herbicide is not commercially acceptable
Results
and Discussion
Most of the chemicals tested
were tolerated by the crops and did not significantly reduce seed yields
in comparison to the untreated weed free check. Everest on seedling
tall fescue, timothy, annual and perennial ryegrass caused significant
and unacceptable visual damage (Tables 1-4). Tall fescue seed yield was
lightly reduced, and both annual and perennial ryegrass seed yield was
significantly reduced in comparison to the untreated weed free check.
However, seed yield for plots sprayed with Everest at the 2x rate had
an average seed yield of 429 kg/ha. This result is not expected and
probably does not reflect the injury and damage that can be caused by
spraying at this rate with this chemical. Actual plot population was
approximately 30-50% less than the rest of the plots. However, the
other plots had a significant amount of residue, which may have
suppressed seed yield, whereas the plots sprayed with Everest at the 2x
rate did not.
Table 1. Tolerance of seedling
tall fescue to herbicide application at Arborg, Manitoba. Plots were
sprayed July 5, 2001. Ratings were taken 3, 14 and 28 days after
treatment (DAT). Plots were harvested for seed July 31, 2002.
|
Treatment |
Crop Tolerance |
Seed Yield (kg/ha) |
|
3 DAT |
14 DAT |
28 DAT |
|
Accord + Merge |
85 |
88 |
100 |
1033 |
|
Attain |
100 |
90 |
100 |
1021 |
|
Buctril M |
100 |
100 |
100 |
1191 |
|
Curtail M 1x |
100 |
95 |
100 |
1417 |
|
Curtail M 2x |
100 |
95 |
100 |
1066 |
|
Everest + MCPA Amine |
77 |
17 |
23 |
932 |
|
Prestige 1x |
98 |
95 |
100 |
1254 |
|
Puma Super 1x |
91 |
83 |
100 |
1049 |
|
Spectrum 1x |
100 |
100 |
100 |
1199 |
|
Spectrum 2x |
98 |
98 |
98 |
1020 |
|
Untreated Weed Free Check |
98 |
100 |
100 |
1137 |
|
LSD |
|
|
|
439.462 |
|
C.V.% |
|
|
|
23.84 |
* Significantly different from untreated weed free check (P=0.05).
Table 2.
Tolerance of seedling perennial ryegrass to herbicide application at
Arborg and St. Norbert, Manitoba. Plots were sprayed July 7 and 10,
2001 respectively. Ratings were taken 3, 14 and 28 days after treatment
(DAT). Plots were harvested for seed August 7, 2002.
|
Treatment |
St. Norbert |
Arborg |
|
Crop Tolerance |
Crop Tolerance |
Seed Yield (kg/ha) |
|
3 DAT |
14 DAT |
28 DAT |
3 DAT |
14 DAT |
28 DAT |
|
Accord 2x |
100 |
100 |
100 |
95 |
91 |
89 |
379 |
|
Assert 1x |
84 |
84 |
98 |
86 |
63 |
93 |
478 |
|
Assert 2x |
75 |
83 |
96 |
84 |
51 |
85 |
442 |
|
Attain 1x |
90 |
88 |
100 |
90 |
75 |
96 |
517 |
|
Attain 2x |
96 |
100 |
100 |
91 |
68 |
96 |
547 |
|
Buctril M 1x |
- |
- |
- |
94 |
100 |
99 |
441 |
|
2, 4 D Ester |
93 |
100 |
100 |
- |
- |
- |
- |
|
Everest 1x |
64 |
19 |
11 |
55 |
5 |
0 |
0* |
|
Everest 2x |
58 |
13 |
6 |
63 |
5 |
1 |
439 |
|
Prestige 1x |
96 |
100 |
100 |
98 |
86 |
99 |
438 |
|
Prestige 2x |
99 |
100 |
100 |
99 |
85 |
96 |
408 |
|
Puma Super 1x |
81 |
96 |
96 |
91 |
84 |
98 |
487 |
|
Puma Super 2x |
61 |
76 |
96 |
85 |
65 |
93 |
420 |
|
Spectrum 1x |
86 |
100 |
100 |
98 |
91 |
100 |
491 |
|
Spectrum 2x |
94 |
93 |
90 |
97 |
88 |
93 |
403 |
|
Untreated Weed Free Control |
100 |
100 |
100 |
100 |
100 |
100 |
444 |
|
LSD |
|
|
|
|
|
|
361.379 |
|
C.V.% |
|
|
|
|
|
|
55.84 |
* Significantly different from untreated weed free check (P=0.05).
Table 3.
Tolerance of annual ryegrass to herbicide application at Arborg and St.
Norbert (Brett Young), Manitoba. Plots at Arborg were sprayed July 5,
2001 and plots at St. Norbert were sprayed July 9, 2001. Ratings were
taken 3, 14 and 28 days after treatment (DAT). Plots were harvested for
seed September 5, 2001.
|
Treatment |
St. Norbert |
Arborg |
|
Crop Tolerance |
Crop Tolerance |
Seed Yield (kg/ha) |
|
3 DAT |
14 DAT |
28 DAT |
3 DAT |
14 DAT |
28 DAT |
|
2, 4 D Ester 700 |
95 |
97 |
97 |
83 |
90 |
100 |
222 |
|
Accord 2x |
97 |
97 |
95 |
92 |
97 |
100 |
270 |
|
Assert 1x |
92 |
100 |
100 |
63 |
93 |
95 |
308 |
|
Assert 2x |
93 |
100 |
95 |
47 |
80 |
93 |
251 |
|
Attain 1x |
92 |
100 |
100 |
100 |
100 |
10 |
340 |
|
Attain 2x |
100 |
100 |
100 |
93 |
100 |
10 |
316 |
|
Everest 1x |
60 |
23 |
53 |
20 |
4 |
1 |
12* |
|
Everest 2x |
52 |
12 |
23 |
13 |
3 |
0 |
0* |
|
Prestige 1x |
97 |
100 |
97 |
90 |
100 |
100 |
368 |
|
Prestige 2x |
98 |
100 |
100 |
100 |
100 |
100 |
315 |
|
Puma Super 1x |
77 |
72 |
92 |
42 |
87 |
92 |
382 |
|
Puma Super 2x |
57 |
28 |
65 |
25 |
45 |
60 |
157* |
|
Spectrum 1x |
92 |
73 |
85 |
88 |
93 |
100 |
276 |
|
Spectrum 2x |
93 |
98 |
100 |
95 |
83 |
100 |
318 |
|
Untreated Weed Free Check |
98 |
100 |
100 |
100 |
97 |
100 |
287 |
|
LSD |
|
|
|
|
|
|
106.2 |
|
C.V.% |
|
|
|
|
|
|
24.92 |
* Significantly different from untreated weed free check (P=0.05).
Table 4.
Tolerance of seedling timothy to herbicide application at Arborg,
Manitoba. Plots were sprayed July 7, 2001 and measurements were taken
3, 14 and 28 days after treatment (DAT).
|
Treatment |
Crop Tolerance |
|
3 DAT |
14 DAT |
28 DAT |
|
Untreated Weed Free Check |
100 |
100 |
100 |
|
Buctril M 1x |
95 |
97 |
100 |
|
Prestige 1x |
97 |
73 |
95 |
|
Prestige 2x |
88 |
83 |
100 |
|
Attain 1x |
97 |
80 |
93 |
|
Attain 2x |
97 |
90 |
100 |
|
Spectrum 1x |
88 |
72 |
97 |
|
Spectrum 2x |
88 |
77 |
95 |
|
Everest + MCPA 1x |
77 |
23 |
78 |
|
Everest + MCPA 2x |
68 |
10 |
30 |
|
Accord 1x |
92 |
65 |
100 |
|
Accord 2x |
97 |
77 |
100 |
|
Achieve |
92 |
97 |
100 |
|
Avenge |
43 |
25 |
72 |
Avenge caused severe initial
damage to seedling timothy (Table 4), however, injuries tended to
outgrow by 28 days after treatment. Timothy plots were not harvested
for seed because plant stand densities were neither high nor consistent
enough to produce reliable results.
Puma Super 2x reduced seed yield
significantly from the untreated weed free check on annual ryegrass at
St. Norbert and had unacceptable tolerance ratings at both sites (Table
3).
Most Refine treatments, as well
as Tropotox and Tropotox Plus 2x treatments were hard on seedling
alfalfa initially (Table 5). However, these injuries were outgrown by
28 days after treatment and didn’t significantly affect seed yield in
comparison to the untreated check.
Table 5. Tolerance of seedling
alfalfa to herbicide application at Arborg, Manitoba. Plots were
sprayed July 6, 2001. Ratings were taken 3, 14 and 28 days after
treatment (DAT). Plots were harvested for seed September 23, 2002.
|
Treatment |
Crop Tolerance |
Seed Yield (kg/ha) |
|
3 DAT |
14 DAT |
28 DAT |
|
Basagran + Assist |
83 |
70 |
79 |
338 |
|
Embutox 1x |
83 |
66 |
91 |
299 |
|
Embutox 2x |
81 |
71 |
84 |
311 |
|
Puma Super 1x |
99 |
86 |
95 |
388 |
|
Puma Super 2x |
98 |
100 |
100 |
279 |
|
Refine 1x + 2, 4 D |
58 |
55 |
93 |
359 |
|
Refine 1x + Agral 90 |
80 |
64 |
95 |
324 |
|
Refine 1x + MCPA Na |
58 |
94 |
78 |
359 |
|
Refine 2x + 2, 4 D |
58 |
55 |
93 |
362 |
|
Refine 2x + Agral 90 |
48 |
79 |
85 |
390 |
|
Refine 2x + MCPA Na |
45 |
89 |
75 |
335 |
|
Refine Extra |
40 |
95 |
50 |
358 |
|
Tropotox 1x |
81 |
71 |
84 |
332 |
|
Tropotox 2x |
60 |
83 |
83 |
325 |
|
Tropotox Plus 1x |
69 |
63 |
96 |
362 |
|
Tropotox Plus 2x |
43 |
94 |
56 |
381 |
|
Venture DG |
87 |
90 |
95 |
301 |
|
Untreated Weed Free Check |
100 |
100 |
100 |
305 |
|
LSD |
|
|
|
96.725 |
|
C.V.% |
|
|
|
20.07 |
* Significantly different from untreated weed free check (P=0.05).
Established Trials:
Procedure and Project
Activities
In 2002, herbicide trials were
conducted on both producers’ fields and on blocks that were seeded the
previous year. Testing was conducted on established perennial ryegrass,
tall fescue, timothy and alfalfa. Seed yields obtained from the timothy
trials were much lower than what was obtained from cooperating producers
therefore they will not be discussed in this report. Lower yields than
expected may be attributed to harvesting and threshing methods.
Established grasses were sprayed before stem elongation and established
alfalfa was sprayed approximately 20 cm in height. Plots were 2m x 6m
and sprayed using a bicycle sprayer. A 10gal/acre (45 L/acre) water
volume was used to spray all treatments. Visual tolerance ratings,
using the scale in Figure 1, were taken approximately 3, 14, and 28-30
days after treatment. An area of 3m2 from each plot was
clipped, bagged, dried and then threshed to obtain seed yield.
Results and Discussion
All herbicides tested on tall
fescue (Table 6) produced very little visual injury and did not decrease
seed yields significantly from the untreated weed free check.
Table 6. Seed yield (kg/ha)
following spring application of herbicide applied at 1x and 2x the
recommended rate on tall fescue and perennial ryegrass in Arborg,
Manitoba .
|
Treatment |
Tall Fescue
(Site 1) |
Tall Fescue
(Site 2) |
Perennial Ryegrass |
|
Accord 1x + 2, 4 D – Ester |
940 |
705 |
333 |
|
Accord 1x + Merge |
1001 |
834 |
345 |
|
Accord 2x + Merge |
1063 |
694 |
306 |
|
Assert 1x |
837 |
1058 |
368 |
|
Assert 2x |
741 |
653 |
264* |
|
Attain 1x |
|
|
541 |
|
Attain 2x |
|
|
417 |
|
Avenge 1x |
726 |
942 |
|
|
Avenge 2x |
869 |
990 |
|
|
Curtail M 1x |
880 |
872 |
|
|
Curtail M 2x |
901 |
822 |
|
|
Prestige 1x |
|
|
533 |
|
Prestige 2x |
|
|
492 |
|
Puma Supra 1x |
|
|
563 |
|
Puma Supra 2x |
|
|
424 |
|
Spectrum 1x |
|
|
480 |
|
Spectrum 2x |
|
|
407 |
|
Target 1x |
1141 |
892 |
|
|
Untreated Weed Free Check |
1137 |
1019 |
398 |
|
LSD |
418.677 |
374.221 |
263.148 |
|
C.V.% |
32.01 |
25.46 |
43.88 |
* Significantly different from untreated weed free check (P=0.05).
Assert lx
was fairly hard on perennial ryegrass but only decreasing yields
slightly, while Assert 2x caused both crop injury and a significant
decrease in seed yield. Accord seemed to have caused some injury at 28
days of treatment, however seed yield was not affected significantly.
Results for established alfalfa
(Table 7) were interesting. Some of the treatments produced severe
injuries to the crop indicated by low ratings for the first two rating
dates. Injuries, severe as they were, tended to outgrow and none of the
treatments produced significantly lower seed yield than the untreated
check.
Table 7. Tolerance of
established alfalfa at Fisher Branch and Arborg, Manitoba. Plots were
sprayed June 4 and June 5, 2002, respectively. Ratings were taken at
varying days after treatment. Plots were harvested for seed October 9
(Fisher Branch) and October 16 (Arborg), 2002.
|
Treatment
|
Fisher Branch |
Arborg |
|
Crop Tolerance |
Seed Yield (kg/ha) |
Crop Tolerance |
Seed Yield (kg/ha) |
|
5 DAT |
14 DAT |
31 DAT |
6 DAT |
14 DAT |
34 DAT |
|
Basagran 1x |
98 |
93 |
99 |
439 |
91 |
98 |
89 |
403 |
|
Embutox 1x |
85 |
78 |
78 |
440 |
62 |
83 |
83 |
391 |
|
Embutox 2x |
63 |
66 |
66 |
439 |
46 |
56 |
75 |
344 |
|
Refine 1x |
73 |
71 |
82 |
452 |
79 |
79 |
84 |
347 |
|
Refine 2x |
70 |
66 |
76 |
416 |
79 |
79 |
86 |
374 |
|
Tropotox 1x |
72 |
74 |
90 |
453 |
76 |
83 |
91 |
354 |
|
Tropotox 2x |
58 |
54 |
75 |
442 |
67 |
71 |
86 |
370 |
|
Tropotox Plus 1x |
85 |
65 |
78 |
416 |
76 |
74 |
91 |
389 |
|
Tropotox Plus 2x |
63 |
56 |
79 |
419 |
78 |
71 |
85 |
364 |
|
Untreated Weed Free
Check |
100 |
100 |
98 |
442 |
100 |
100 |
96 |
358 |
|
LSD |
|
|
|
85.742 |
|
|
|
68.214 |
|
C.V.% |
|
|
|
15.41 |
|
|
|
12.72 |
* Significantly different from untreated weed free check (P=0.05).
Conclusions:
The harmful effect of Everest on
forage seed grasses is an important result obtained from testing. It
was hoped that it would become a product that could be used for
controlling wild oats and green foxtail in several grass seed crops.
Data from these trials has been used to help support minor use
registrations. Recent minor use registrations include:
-
Attain on seedling and
established tall fescue - obtained in February, 2002.
-
Prestige on established tall
fescue - obtained in July, 2002.
-
Tropotox Plus on seedling
alfalfa - obtained February, 2003.
Applications have been made to
include established Timothy (seed and forage) on Prestige, Spectrum and
Curtail M labels.
The federal government is
currently in the process of developing a new minor use program to
facilitate registration. They are in the process of developing a
national priority list of products and crop kinds that will receive
complete funding to pay for the research and personnel required to
obtain registration. Results obtained from this and previous years’
research has been vital in determining products and crops to propose for
this list. Results obtained will be used to obtain registrations for
products not on the national priority list. The Manitoba Forage Seed
Association plans to continue working with Alberta, British Columbia,
and Saskatchewan on obtaining minor uses for forage seed crops and
screening new herbicides that come on the market.
Acknowledgement:
We wish to thank the following
producers and seed companies for their participation in the forage seed
herbicide program: Lorne Johnson, Bill Mamchuk, Len and Linda Loewen,
Brian and Eric Fridfinnson, Johnson Seeds and Brett-Young Seeds. We are
grateful for the technical assistance provided by Anthony Mintenko
(Manitoba Agriculture, Food and Rural Initiatives), Sydney Ramnath,
Jennifer Blandford, and Kendell Malenchak.
Funding for this research was
provided by the Agri-Food Research and Development Initiative (ARDI).
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