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Manitoba Agriculture, Food and Rural Initiatives

PROJECT RESULTS

 

Forage and Forage Seed Crop Herbicide/Weed Control Evaluation Program

 

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Applicant: 

Michelle Watts

Manitoba Forage Seed Association

Arborg, Manitoba  R0C 0A0  Canada

 

Table of Contents:

 

ARDI Project:

 

#00-416

Total Approved:

$30,000

Date Approved:

April 9, 2001

Project Status:

Completed March, 2003

 

Background and Objectives:

There were over 110,000 acres of forage seed produced in Manitoba in 2000.  Registered options available to both forage and forage seed growers remain limited causing a high rate of unregistered pesticide use in both industries.  To obtain a User Requested Minor Use Label Expansion (URMULE), the Pest Management Regulatory Agency requires at least 2-3 years of research that includes crop tolerance and impacts on seed yield.  Weed control issues have been identified as the number one limitation to increasing seed yield and quality in forage and grass seed crops across Manitoba.  Development of effective and economical weed control programs is necessary for the industry to continue growing and maintain stability.

The Manitoba Forage Seed Association conducted a broad-spectrum herbicide tolerance screening trial in 2000.  Results from this trial were used to determine products to pursue for registration.  Trials in 2001 and 2002 were coordinated with government specialists from British Columbia, Alberta, and Saskatchewan.  Both seed trade and producers from western Canada were consulted and provided valuable input.  Trials in 2001 focused on seedling crops while trials in 2002 targeted established crops.  Herbicides tested have a determined weed spectrum and a major registration.  Treatments included products to address both grassy and broad leaf weed problems such as cleavers, wild oats, green foxtail barnyard grass, Canada thistle, perennial sow-thistle, dandelion, as well as others in forage seed crops.

Seedling Trials:

Procedure and Project Activities

In 2001, large blocks of forage seed crops were solid seeded in Arborg and St. Norbert, Manitoba to conduct herbicide trials.  Species seeded included alfalfa (Medicago sativa), perennial ryegrass (Lolium perenne), timothy (Phleum pratense), tall fescue (Festuca arundinacea) and annual ryegrass (Lolium multiflorum).  All species were successfully established at Arborg while only perennial and annual ryegrasses were successfully established at St. Norbert.  Seedling grasses were sprayed at the 2-4 leaf stage and seedling alfalfa was sprayed at the 3-4 trifoliate stage.  Crop tolerance ratings, using the scale in Figure 1, were taken approximately 3, 14 and 28 days after treatment.  Plots were 2m x 6m and were sprayed with a bicycle sprayer.  A 10g/acre (45L/acre) water volume was used to spray all treatments.  An area of 3m2 from each plot was clipped, bagged, dried and threshed to obtain seed yields.  The annual ryegrass plot in Arborg was not harvested for seed yield due to a flooding period, which affected fertility and seed production.  As well, the timothy plot in Arborg was not taken to seed yield to due poor establishment.

Figure 1.  0-100% Crop Tolerance Ratings

100

Complete Tolerance

90-80

Possible Effect

70

Slight Effect

60

Definite Effect

50-10

Severe Effect

0

Complete Kill

< 70% crop tolerance to herbicide is not commercially acceptable

 

Results and Discussion

Most of the chemicals tested were tolerated by the crops and did not significantly reduce seed yields in comparison to the untreated weed free check.  Everest on seedling tall fescue, timothy, annual and perennial ryegrass caused significant and unacceptable visual damage (Tables 1-4).  Tall fescue seed yield was lightly reduced, and both annual and perennial ryegrass seed yield was significantly reduced in comparison to the untreated weed free check.  However, seed yield for plots sprayed with Everest at the 2x rate had an average seed yield of 429 kg/ha.  This result is not expected and probably does not reflect the injury and damage that can be caused by spraying at this rate with this chemical.  Actual plot population was approximately 30-50% less than the rest of the plots.  However, the other plots had a significant amount of residue, which may have suppressed seed yield, whereas the plots sprayed with Everest at the 2x rate did not.

Table 1.  Tolerance of seedling tall fescue to herbicide application at Arborg, Manitoba.  Plots were sprayed July 5, 2001.  Ratings were taken 3, 14 and 28 days after treatment (DAT).  Plots were harvested for seed July 31, 2002.

Treatment

Crop Tolerance

Seed Yield (kg/ha)

3 DAT

14 DAT

28 DAT

Accord + Merge

85

88

100

1033

Attain

100

90

100

1021

Buctril M

100

100

100

1191

Curtail M 1x

100

95

100

1417

Curtail M 2x

100

95

100

1066

Everest + MCPA Amine

77

17

23

932

Prestige 1x

98

95

100

1254

Puma Super 1x

91

83

100

1049

Spectrum 1x

100

100

100

1199

Spectrum 2x

98

98

98

1020

Untreated Weed Free Check

98

100

100

1137

LSD

 

 

 

439.462

C.V.%

 

 

 

23.84

* Significantly different from untreated weed free check (P=0.05).

 

Table 2.  Tolerance of seedling perennial ryegrass to herbicide application at Arborg and St. Norbert, Manitoba.  Plots were sprayed July 7 and 10, 2001 respectively.  Ratings were taken 3, 14 and 28 days after treatment (DAT).  Plots were harvested for seed August 7, 2002.

Treatment

St. Norbert

Arborg

Crop Tolerance

Crop Tolerance

Seed Yield (kg/ha)

3 DAT

14 DAT

28 DAT

3 DAT

14 DAT

28 DAT

Accord 2x

100

100

100

95

91

89

379

Assert 1x

84

84

98

86

63

93

478

Assert 2x

75

83

96

84

51

85

442

Attain 1x

90

88

100

90

75

96

517

Attain 2x

96

100

100

91

68

96

547

Buctril M 1x

-

-

-

94

100

99

441

2, 4 D Ester

93

100

100

-

-

-

-

Everest 1x

64

19

11

55

5

0

0*

Everest 2x

58

13

6

63

5

1

439

Prestige 1x

96

100

100

98

86

99

438

Prestige 2x

99

100

100

99

85

96

408

Puma Super 1x

81

96

96

91

84

98

487

Puma Super 2x

61

76

96

85

65

93

420

Spectrum 1x

86

100

100

98

91

100

491

Spectrum 2x

94

93

90

97

88

93

403

Untreated Weed Free Control

100

100

100

100

100

100

444

LSD

 

 

 

 

 

 

361.379

C.V.%

 

 

 

 

 

 

55.84

* Significantly different from untreated weed free check (P=0.05).

 

Table 3.  Tolerance of annual ryegrass to herbicide application at Arborg and St. Norbert (Brett Young), Manitoba.  Plots at Arborg were sprayed July 5, 2001 and plots at St. Norbert were sprayed July 9, 2001.  Ratings were taken 3, 14 and 28 days after treatment (DAT).  Plots were harvested for seed September 5, 2001. 

Treatment

St. Norbert

Arborg

Crop Tolerance

Crop Tolerance

Seed Yield (kg/ha)

3 DAT

14 DAT

28 DAT

3 DAT

14 DAT

28 DAT

2, 4 D Ester 700

95

97

97

83

90

100

222

Accord 2x

97

97

95

92

97

100

270

Assert 1x

92

100

100

63

93

95

308

Assert 2x

93

100

95

47

80

93

251

Attain 1x

92

100

100

100

100

10

340

Attain 2x

100

100

100

93

100

10

316

Everest 1x

60

23

53

20

4

1

12*

Everest 2x

52

12

23

13

3

0

0*

Prestige 1x

97

100

97

90

100

100

368

Prestige 2x

98

100

100

100

100

100

315

Puma Super 1x

77

72

92

42

87

92

382

Puma Super 2x

57

28

65

25

45

60

157*

Spectrum 1x

92

73

85

88

93

100

276

Spectrum 2x

93

98

100

95

83

100

318

Untreated Weed Free Check

98

100

100

100

97

100

287

LSD

 

 

 

 

 

 

106.2

C.V.%

 

 

 

 

 

 

24.92

* Significantly different from untreated weed free check (P=0.05).

 

Table 4. Tolerance of seedling timothy to herbicide application at Arborg, Manitoba.  Plots were sprayed July 7, 2001 and measurements were taken 3, 14 and 28 days after treatment (DAT). 

Treatment

Crop Tolerance

3 DAT

14 DAT

28 DAT

Untreated Weed Free Check

100

100

100

Buctril M 1x

95

97

100

Prestige 1x

97

73

95

Prestige 2x

88

83

100

Attain 1x

97

80

93

Attain 2x

97

90

100

Spectrum 1x

88

72

97

Spectrum 2x

88

77

95

Everest + MCPA 1x

77

23

78

Everest + MCPA 2x

68

10

30

Accord 1x

92

65

100

Accord 2x

97

77

100

Achieve

92

97

100

Avenge

43

25

72

 

Avenge caused severe initial damage to seedling timothy (Table 4), however, injuries tended to outgrow by 28 days after treatment.  Timothy plots were not harvested for seed because plant stand densities were neither high nor consistent enough to produce reliable results.

Puma Super 2x reduced seed yield significantly from the untreated weed free check on annual ryegrass at St. Norbert and had unacceptable tolerance ratings at both sites (Table 3).

Most Refine treatments, as well as Tropotox and Tropotox Plus 2x treatments were hard on seedling alfalfa initially (Table 5).  However, these injuries were outgrown by 28 days after treatment and didn’t significantly affect seed yield in comparison to the untreated check.

Table 5.  Tolerance of seedling alfalfa to herbicide application at Arborg, Manitoba.  Plots were sprayed July 6, 2001.  Ratings were taken 3, 14 and 28 days after treatment (DAT).  Plots were harvested for seed September 23, 2002. 

Treatment

Crop Tolerance

Seed Yield (kg/ha)

3 DAT

14 DAT

28 DAT

Basagran + Assist

83

70

79

338

Embutox 1x

83

66

91

299

Embutox 2x

81

71

84

311

Puma Super 1x

99

86

95

388

Puma Super 2x

98

100

100

279

Refine 1x + 2, 4 D

58

55

93

359

Refine 1x + Agral 90

80

64

95

324

Refine 1x + MCPA Na

58

94

78

359

Refine 2x + 2, 4 D

58

55

93

362

Refine 2x + Agral 90

48

79

85

390

Refine 2x + MCPA Na

45

89

75

335

Refine Extra

40

95

50

358

Tropotox 1x

81

71

84

332

Tropotox 2x

60

83

83

325

Tropotox Plus 1x

69

63

96

362

Tropotox Plus 2x

43

94

56

381

Venture DG

87

90

95

301

Untreated Weed Free Check

100

100

100

305

LSD

 

 

 

96.725

C.V.%

 

 

 

20.07

* Significantly different from untreated weed free check (P=0.05).

 

Established Trials:

Procedure and Project Activities

In 2002, herbicide trials were conducted on both producers’ fields and on blocks that were seeded the previous year.  Testing was conducted on established perennial ryegrass, tall fescue, timothy and alfalfa.  Seed yields obtained from the timothy trials were much lower than what was obtained from cooperating producers therefore they will not be discussed in this report.  Lower yields than expected may be attributed to harvesting and threshing methods.  Established grasses were sprayed before stem elongation and established alfalfa was sprayed approximately 20 cm in height.  Plots were 2m x 6m and sprayed using a bicycle sprayer.  A 10gal/acre (45 L/acre) water volume was used to spray all treatments.  Visual tolerance ratings, using the scale in Figure 1, were taken approximately 3, 14, and 28-30 days after treatment.  An area of 3m2 from each plot was clipped, bagged, dried and then threshed to obtain seed yield.

Results and Discussion

All herbicides tested on tall fescue (Table 6) produced very little visual injury and did not decrease seed yields significantly from the untreated weed free check.

Table 6.  Seed yield (kg/ha) following spring application of herbicide applied at 1x and 2x the recommended rate on tall fescue and perennial ryegrass in Arborg, Manitoba .

Treatment

Tall Fescue

(Site 1)

Tall Fescue

(Site 2)

Perennial Ryegrass

Accord 1x + 2, 4 D – Ester

940

705

333

Accord 1x + Merge

1001

834

345

Accord 2x + Merge

1063

694

306

Assert 1x

837

1058

368

Assert 2x

741

653

264*

Attain 1x

 

 

541

Attain 2x

 

 

417

Avenge 1x

726

942

 

Avenge 2x

869

990

 

Curtail M 1x

880

872

 

Curtail M 2x

901

822

 

Prestige 1x

 

 

533

Prestige 2x

 

 

492

Puma Supra 1x

 

 

563

Puma Supra 2x

 

 

424

Spectrum 1x

 

 

480

Spectrum 2x

 

 

407

Target 1x

1141

892

 

Untreated Weed Free Check

1137

1019

398

LSD

418.677

374.221

263.148

C.V.%

32.01

25.46

43.88

* Significantly different from untreated weed free check (P=0.05).

 

Assert lx was fairly hard on perennial ryegrass but only decreasing yields slightly, while Assert 2x caused both crop injury and a significant decrease in seed yield. Accord seemed to have caused some injury at 28 days of treatment, however seed yield was not affected significantly.

Results for established alfalfa (Table 7) were interesting.  Some of the treatments produced severe injuries to the crop indicated by low ratings for the first two rating dates. Injuries, severe as they were, tended to outgrow and none of the treatments produced significantly lower seed yield than the untreated check.

Table 7.  Tolerance of established alfalfa at Fisher Branch and Arborg, Manitoba.  Plots were sprayed June 4 and June 5, 2002, respectively.  Ratings were taken at varying days after treatment.  Plots were harvested for seed October 9 (Fisher Branch) and October 16 (Arborg), 2002. 

Treatment

 

 

Fisher Branch

Arborg

Crop Tolerance

Seed Yield (kg/ha)

Crop Tolerance

Seed Yield (kg/ha)

5 DAT

14 DAT

31 DAT

6 DAT

14 DAT

34 DAT

Basagran 1x

98

93

99

439

91

98

89

403

Embutox 1x

85

78

78

440

62

83

83

391

Embutox 2x

63

66

66

439

46

56

75

344

Refine 1x

73

71

82

452

79

79

84

347

Refine 2x

70

66

76

416

79

79

86

374

Tropotox 1x

72

74

90

453

76

83

91

354

Tropotox 2x

58

54

75

442

67

71

86

370

Tropotox Plus 1x

85

65

78

416

76

74

91

389

Tropotox Plus 2x

63

56

79

419

78

71

85

364

Untreated Weed Free Check

100

100

98

442

100

100

96

358

LSD

 

 

 

85.742

 

 

 

68.214

C.V.%

 

 

 

15.41

 

 

 

12.72

* Significantly different from untreated weed free check (P=0.05).

 

Conclusions:

The harmful effect of Everest on forage seed grasses is an important result obtained from testing.  It was hoped that it would become a product that could be used for controlling wild oats and green foxtail in several grass seed crops.  Data from these trials has been used to help support minor use registrations.  Recent minor use registrations include:

  1. Attain on seedling and established tall fescue - obtained in February, 2002.

  2. Prestige on established tall fescue - obtained in July, 2002.

  3. Tropotox Plus on seedling alfalfa - obtained February, 2003.

Applications have been made to include established Timothy (seed and forage) on Prestige, Spectrum and Curtail M labels.

The federal government is currently in the process of developing a new minor use program to facilitate registration.  They are in the process of developing a national priority list of products and crop kinds that will receive complete funding to pay for the research and personnel required to obtain registration.  Results obtained from this and previous years’ research has been vital in determining products and crops to propose for this list.  Results obtained will be used to obtain registrations for products not on the national priority list.  The Manitoba Forage Seed Association plans to continue working with Alberta, British Columbia, and Saskatchewan on obtaining minor uses for forage seed crops and screening new herbicides that come on the market.

Acknowledgement:

We wish to thank the following producers and seed companies for their participation in the forage seed herbicide program: Lorne Johnson, Bill Mamchuk, Len and Linda Loewen, Brian and Eric Fridfinnson, Johnson Seeds and Brett-Young Seeds.  We are grateful for the technical assistance provided by Anthony Mintenko (Manitoba Agriculture, Food and Rural Initiatives), Sydney Ramnath, Jennifer Blandford, and Kendell Malenchak.

Funding for this research was provided by the Agri-Food Research and Development Initiative (ARDI).

 

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