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Manitoba Agriculture, Food and Rural Initiatives

PROJECT RESULTS

 

Genetic Improvement of Canola

 

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Applicant: 

Dr. Radisa Gjuric

Brett-Young Seeds Limited

Winnipeg, Manitoba  R3V 1L5  Canada

 

Table of Contents:

 

 

ARDI Project:

 

#01-485

Total Approved: $600,000
Date Approved: October 11, 2001

Project Status:

Completed January, 2005

 

Background and Objective:

Hybrid canola is anticipated to comprise the large majority of the canola acres planted in the near future.  The successful exploitation of heterosis in canola is a key to increasing value of the canola crop to growers.  The majority of the hybrid canola seed production is currently based in irrigated areas of Southern Alberta and world oil markets are increasingly demanding healthier oils.

The objectives of the program were:

  1. Develop high yielding canola hybrid varieties in a hybrid system(s) that have no problem with temperature sensitivity.

  2. Develop the basic concepts of gene pools and explore heterosis in canola.

  3. Develop a method of fast conversion of modified oil canola accessions into germplasm carrying traits that are adapted to the Western Canadian market.

Procedure and Project Activities:

Objective 1 – Hybrid Development

Initial material for hybrid development was accessed from public and private breeding organizations.  Male sterile lines and restorer lines were developed and evaluated in various hybrid combinations.

Objective 2 – Heterosis in Canola

A series of diallel crosses were performed from a diverse background.  Hybrid combinations were evaluated for yield to establish heterotic effects.

Objective 3 – Modified Oil Conversion

A number of accessions with modified oil profiles were evaluated with a molecular marker backcrossing scheme for conversion of improved germplasm to the modified oil profile.

Results and Discussion:

Objective 1 – Hybrid Development

New restorer and mother line development is presented in Tables 1.1 and 1.2.  The number of test hybrid combinations tested in trials (Table 1.4) is presented in Table 1.3.

Table 1.1.  New Restorer Development

Nursery Plots

2000

2001

2002

2003

Rf NHT

 

 

 

 

 

108

270

329

250

 

62

40

0

0

Rf RR

 

 

 

 

 

124

300

1054

304

 

152

130

28

85

Rf CF

 

 

 

 

 

11

30

59

30

Other plots (AxB, etc..)

 

 

 

1331

 

Table 1.2.  New Mother Line Development

  2000 2001 2002 2003
Ogura CMS mother lines increased to > 5g 80 205 105 132

 

Table 1.3  Number of Test Hybrids Developed and Tested During 2000-2003

 

2000

2001

2002

2003

Ogura Hybrids

71

0

142

0

MSL Hybrids

16

16

0

40

NHT Hybrids

33

0

0

0

IMI Hybrids

0

0

18

0

RR Hybrids

17

320

371

45

Total Hybrids

137

336

531

85

 

Table 1.4.  Number of Trial Plots on All Locations (Private and In-House Trials)

 

2000

2001

2002

2003

Yield Trials

4320

7050

9458

8294

Blackleg Trials

1800

3690

2390

1692

 

Objective 1 was met and the timetable was followed.  We have developed hybrids in Ogura hybrid system and all the indications are that production will not be affected by stability under high temperatures.  The restorer lines were developed in-house, therefore no need to enter licensing agreements with patent holders, i.e. Pioneer Hi-Bred and Monsanto.  Five hybrids recommended for registration (4 in 2004 and 1 in 2005) were developed with the help of ARDI funds. 

Newer mother lines for MSL hybrids did not show significant improvement and this part was dropped to provide resources for work on Ogura system.  Decision tools used are based on statistical analysis of field data.

Facilities and equipment provided by ARDI funds allowed us to increase the volume of a) field testing, b) start in-house double haploid production, c) increase the volume of greenhouse program; all of which contributed to successful completion of this objective.

Objective 2 – Heterosis in Canola

A study was organized in 1001-2002 to understand the concept of geographic gene pools.  Results are presented in table 2.1.  These results showed that gene pools of geographic origin are a poor predictor of heterosis.

Table 2.1.

MSL

 

POL

Data

MIX 501

MIX 540

EU 541

EU 542

EU 543

AUS 544

Total

Aus1

KGHA

1138.0

1208.5

1224.0

1159.0

1249.9

1378.1

1226.3

OIL

43.9

44.2

44.6

44.6

43.8

44.6

44.3

PROT

49.6

49.4

49.0

48.8

50.0

47.9

49.1

GSL

17.5

18.4

17.2

19.1

19.2

17.9

18.2

Aus2

KGHA

1533.5

1474.9

1503.1

1362.4

1385.3

1689.3

1491.4

OIL

43.4

44.8

43.2

42.6

43.3

43.8

43.5

PROT

48.6

47.8

48.0

49.8

48.2

47.5

48.3

GSL

24.2

22.6

23.0

29.5

27.3

24.5

25.2

Aus3

KGHA

1486.2

1464.2

1394.0

1364.5

1573.0

1328.0

1435.0

OIL

45.0

44.2

43.8

44.4

44.6

44.0

44.3

PROT

48.1

48.4

48.1

48.2

47.6

47.7

48.0

GSL

14.8

20.1

17.6

18.8

18.4

15.5

17.5

Aus4

KGHA

1116.9

1049.8

1341.3

1115.5

1376.0

1496.6

1249.4

OIL

44.5

44.5

44.3

43.8

45.7

44.2

44.5

PROT

48.5

49.2

48.6

48.4

47.7

47.9

48.4

GSL

16.1

17.6

17.0

17.6

14.8

16.5

16.6

Aus5

KGHA

1373.5

1666.7

1512.5

1305.8

1582.8

1755.8

1532.9

OIL

43.6

44.5

43.7

43.1

44.4

43.3

43.8

PROT

48.3

47.8

47.8

47.6

47.3

47.5

47.7

GSL

17.4

16.6

18.1

19.8

16.3

17.8

17.7

Aus6

KGHA

1439.4

1387.5

1379.2

1155.9

1420.3