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Background and
Objective:
Hybrid canola is anticipated to comprise the large
majority of the canola acres planted in the near future. The successful
exploitation of heterosis in canola is a key to increasing value of the
canola crop to growers. The majority of the hybrid canola seed
production is currently based in irrigated areas of Southern Alberta and
world oil markets are increasingly demanding healthier oils.
The objectives of the program were:
-
Develop high yielding canola hybrid varieties in a
hybrid system(s) that have no problem with temperature sensitivity.
-
Develop the basic concepts of gene pools and
explore heterosis in canola.
-
Develop a method of fast conversion of modified oil
canola accessions into germplasm carrying traits that are adapted to
the Western Canadian market.
Procedure and Project
Activities:
Objective 1 – Hybrid Development
Initial material for hybrid
development was accessed from public and private breeding
organizations. Male sterile lines and restorer lines were developed and
evaluated in various hybrid combinations.
Objective 2 – Heterosis in
Canola
A series of diallel crosses were
performed from a diverse background. Hybrid combinations were evaluated
for yield to establish heterotic effects.
Objective 3 – Modified Oil
Conversion
A number of accessions with
modified oil profiles were evaluated with a molecular marker
backcrossing scheme for conversion of improved germplasm to the modified
oil profile.
Results
and Discussion:
Objective 1 – Hybrid Development
New restorer and mother line development is
presented in Tables 1.1 and 1.2. The number of test hybrid combinations
tested in trials (Table 1.4) is presented in Table 1.3.
Table 1.1. New Restorer
Development
|
Nursery Plots |
2000 |
2001 |
2002 |
2003 |
|
Rf NHT |
|
|
|
|
|
|
108 |
270 |
329 |
250 |
|
|
62 |
40 |
0 |
0 |
|
Rf RR |
|
|
|
|
|
|
124 |
300 |
1054 |
304 |
|
|
152 |
130 |
28 |
85 |
|
Rf CF |
|
|
|
|
|
|
11 |
30 |
59 |
30 |
|
Other plots (AxB, etc..) |
|
|
|
1331 |
Table 1.2. New Mother
Line Development
|
|
2000 |
2001 |
2002 |
2003 |
|
Ogura CMS mother lines increased to > 5g |
80 |
205 |
105 |
132 |
Table 1.3 Number of Test
Hybrids Developed and Tested During 2000-2003
|
|
2000 |
2001 |
2002 |
2003 |
|
Ogura Hybrids |
71 |
0 |
142 |
0 |
|
MSL Hybrids |
16 |
16 |
0 |
40 |
|
NHT Hybrids |
33 |
0 |
0 |
0 |
|
IMI Hybrids |
0 |
0 |
18 |
0 |
|
RR Hybrids |
17 |
320 |
371 |
45 |
|
Total Hybrids |
137 |
336 |
531 |
85 |
Table
1.4. Number of Trial Plots on All Locations (Private and In-House
Trials)
|
|
2000 |
2001 |
2002 |
2003 |
|
Yield Trials |
4320 |
7050 |
9458 |
8294 |
|
Blackleg Trials |
1800 |
3690 |
2390 |
1692 |
Objective 1 was met and the timetable
was followed. We have developed hybrids in Ogura hybrid system and all
the indications are that production will not be affected by stability
under high temperatures. The restorer lines were developed in-house,
therefore no need to enter licensing agreements with patent holders,
i.e. Pioneer Hi-Bred and Monsanto. Five hybrids recommended for
registration (4 in 2004 and 1 in 2005) were developed with the help of
ARDI funds.
Newer mother lines for MSL hybrids did
not show significant improvement and this part was dropped to provide
resources for work on Ogura system. Decision tools used are based on
statistical analysis of field data.
Facilities and equipment provided by
ARDI funds allowed us to increase the volume of a) field testing, b)
start in-house double haploid production, c) increase the volume of
greenhouse program; all of which contributed to successful completion of
this objective.
Objective 2 – Heterosis in Canola
A study was organized in 1001-2002 to understand
the concept of geographic gene pools. Results are presented in table
2.1. These results showed that gene pools of geographic origin are a
poor predictor of heterosis.
Table 2.1.
|
MSL |
|
|
POL |
Data |
MIX 501 |
MIX 540 |
EU 541 |
EU 542 |
EU 543 |
AUS 544 |
Total |
|
Aus1 |
KGHA |
1138.0 |
1208.5 |
1224.0 |
1159.0 |
1249.9 |
1378.1 |
1226.3 |
|
OIL |
43.9 |
44.2 |
44.6 |
44.6 |
43.8 |
44.6 |
44.3 |
|
PROT |
49.6 |
49.4 |
49.0 |
48.8 |
50.0 |
47.9 |
49.1 |
|
GSL |
17.5 |
18.4 |
17.2 |
19.1 |
19.2 |
17.9 |
18.2 |
|
Aus2 |
KGHA |
1533.5 |
1474.9 |
1503.1 |
1362.4 |
1385.3 |
1689.3 |
1491.4 |
|
OIL |
43.4 |
44.8 |
43.2 |
42.6 |
43.3 |
43.8 |
43.5 |
|
PROT |
48.6 |
47.8 |
48.0 |
49.8 |
48.2 |
47.5 |
48.3 |
|
GSL |
24.2 |
22.6 |
23.0 |
29.5 |
27.3 |
24.5 |
25.2 |
|
Aus3 |
KGHA |
1486.2 |
1464.2 |
1394.0 |
1364.5 |
1573.0 |
1328.0 |
1435.0 |
|
OIL |
45.0 |
44.2 |
43.8 |
44.4 |
44.6 |
44.0 |
44.3 |
|
PROT |
48.1 |
48.4 |
48.1 |
48.2 |
47.6 |
47.7 |
48.0 |
|
GSL |
14.8 |
20.1 |
17.6 |
18.8 |
18.4 |
15.5 |
17.5 |
|
Aus4 |
KGHA |
1116.9 |
1049.8 |
1341.3 |
1115.5 |
1376.0 |
1496.6 |
1249.4 |
|
OIL |
44.5 |
44.5 |
44.3 |
43.8 |
45.7 |
44.2 |
44.5 |
|
PROT |
48.5 |
49.2 |
48.6 |
48.4 |
47.7 |
47.9 |
48.4 |
|
GSL |
16.1 |
17.6 |
17.0 |
17.6 |
14.8 |
16.5 |
16.6 |
|
Aus5 |
KGHA |
1373.5 |
1666.7 |
1512.5 |
1305.8 |
1582.8 |
1755.8 |
1532.9 |
|
OIL |
43.6 |
44.5 |
43.7 |
43.1 |
44.4 |
43.3 |
43.8 |
|
PROT |
48.3 |
47.8 |
47.8 |
47.6 |
47.3 |
47.5 |
47.7 |
|
GSL |
17.4 |
16.6 |
18.1 |
19.8 |
16.3 |
17.8 |
17.7 |
|
Aus6 |
KGHA |
1439.4 |
1387.5 |
1379.2 |
1155.9 |
1420.3 |
| |