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Background
and Objective:
Today, Manitoba has become a leader in both legume seed and leafcutter bee production.
In the early 1970s, this industry was virtually non-existent. As development
occurred, successful production systems were developed through a cooperative industry
approach. Alfalfa and other legume seed growers continue to struggle with perennial weeds.
The forage seed industry has repeatedly identified this as a top priority. The yield
damaging effects of perennial weeds on these crops is estimated at 20-25%. By reducing
this effect by even 50%, both the increased farm gate value and increased productivity
would be approximately 2 million dollars. This project looked at increasing effective
registered herbicide options to enhance Manitobas legume seed production industry.
Procedure and Project Activities:
Year 1 (1998)
In 1998, herbicide trials on seedling forage seed crops were planned. Due to
circumstances, plots were seeded late and did not establish well enough to continue the
tests.
Year 2 (1999)
In 1999, herbicide effect on weed control in established alfalfa seed crops was examined.
Herbicide treatments were applied with a bicycle sprayer and plots were located on
commercial fields. Plots were 2 meters by 5 meters in size and treatments were replicated
four times. The sprayer boom was equipped with a two-meter boom with 4 nozzles (8001) at
50-cm spacing. A pressure of 45 psi and a speed of 5 km/hr (3 mph) were used when
spraying. The plots were sprayed with Matador on June 17, 1999, and with herbicide
treatments on June 18, 1999. Herbicide treatments included:
- Control (no chemical applied)
- Basagran at 0.9 L/acre
- Roundup at 0.5 L/acre
- 2,4-DB (Embutox) at 0.9 L/acre
- MCPA Na (sodium salt) at 1.7 L/acre
- MCPB + MVPA (Tropotox plus) at 1.7 L/acre
- MCPB (Tropotox)
- MCPB (Tropotox) + Pardner at 1.7 and 0.5 L/acre
- MCPA Na (sodium salt) + Pardner at 0.4 and 0.5 L/acre
- 2,4 DB (Embutox) + Pardner at 0.9 and 0.5 L/acre
Plots were harvested October 12, 1999, by hand clipping ¼-meter squares (50 cm x 50
cm) from the front and back of each plot. This is not the most ideal method of harvesting
as variability is quite high. Yields were ranked as follows, from highest to lowest:
- MCPB Tropotox
- Tropotox
- MCPA Na
- Tropotox + Pardner
- Embutox + Pardner
- Embutox
- MCPA Na + Pardner
- Roundup
- Basagran
Weed control was quite good in spite of the herbicides being applied a little later
than the optimum window; however, crop tolerance was an issue with all chemicals. Alfalfa
seed producers may have to put up with some crop damage in order to control weeds, as most
herbicides used in alfalfa seed caused some crop damage. Given the low tolerance for
certain weed seeds in alfalfa seed and that many of the weeds targeted are perennials,
yield may have to be sacrificed some degree to eliminate certain weed species.
In the 1999 season, we also planned to have herbicide screening trials on seedling
alfalfa, birdsfoot trefoil, red clover, alsike clover and sweet clover in two locations in
Manitoba. Once the crops were established, these screening trials would be continued for
the next two years to evaluate the herbicides. However, test plots did not get well
established and could not be sprayed as crop growth was too variable to be able to compare
the treatments. Problems were due to soil crusting at one site and wet soil conditions at
the other.
Year 3 (2000)
In 2000, a number of herbicides were screened over a number of forage seed legume species
to identify crop tolerance to herbicide products and to observe effect on seed yield and
quality.
Seedling Tolerance Screening Trials
Seedling tolerance screening trials were conducted at Arborg, MB in 2000 on 6 legume
crops: Algonquin alfalfa, Vector alfalfa, Leo Birdsfoot trefoil, red clover, Norgold sweet
clover and Aurora Alsike clover. Twenty-three herbicide products and 1 untreated control
were used in the trial (Table 1). This trial was initiated at two sites, but one had to be
abandoned due to poor emergence as a result of adverse weather conditions.
Table 1. Treatments for seedling herbicide
screening trial.
|
Species/Cultivar |
Herbicide |
Rate/acre |
Water Volume/acre |
|
Algonquin Alfalfa |
Compas/Amigo |
0.08 L/ac + .5 L per 100 solution |
45 L |
|
Vector Alfalfa |
Pardner |
.405 L |
70 L |
|
Leo Birdsfoot Trefoil |
Pardner +MCPA na+ |
.405 L/.36 L |
90 L |
|
Red Clover |
MCPB |
1.11 L |
90 L |
|
Norgold Sweet Clover |
Curtail M |
.80 L |
45 L |
|
Aurora Alsike Clover |
Assert |
.34 L |
45 L |
|
|
Pursuit/Agsurf |
.085 L/.25 L per 100L of solution |
180 L |
|
|
Odyssey |
.012 kg |
45 L |
|
|
Attain Pack |
A @ .24 L/ B @ .40/L |
45 L |
|
|
Prestige A & B |
.32 L/.8 L |
45 L |
|
|
Puma Super |
.308 L |
45 L |
|
|
Everest |
17.4 g |
45 L |
|
|
Prevail |
4 Package 20 acres |
45 L |
|
|
Horizon/Score |
.095 L/.8 L per 100 L spray solution |
45 L |
|
|
Accord |
.054 kg |
45 L |
|
|
Target |
.40 L |
45 L |
|
|
Avenge |
1.42 L |
45 L |
|
|
Prism/Agsurf |
.024 kg/.2 L per 100 L spray solution |
45 L |
|
|
Pinnacle/Agsurf |
2.2 g/1 L per 1000 L of spray |
45 L |
|
|
Accent/Agsurf |
.0135 kg/.2 L per 100 L of solution |
45 L |
|
|
Embutox 625 |
.71 L |
90 L |
|
|
Atrazine 480/oil concentrate |
.45 kg/1.1 L |
135 L |
|
|
Buctril M |
.405 L |
70 L |
|
|
Untreated Control |
|
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The purpose of the seedling trial was to identify herbicides with potential to control
both grass and broadleaf weeds during establishment of forage seed crops (Table 2).
Herbicide replicated screening trial plot size was 2m x 2m. The trial was seeded on May
26 and the herbicide treatments were applied on July 6, 2000.
Visual assessments were conducted at 5, 10 41 days after treatment (DAT).
Herbicide tolerance ratings were done numerically on a scale of 0-9. For crop tolerance, 0
would mean complete kill of the crop while a rating of 9 would be complete tolerance.
Water volumes were the maximum recommended for each herbicide.
Established Trials
The established trials consisted of two established legume species: alfalfa and birdsfoot
trefoil. Plot size was 8m x 2m. On each trial site a total of 10 different treatments and
1 untreated control were applied (Table 3). These treatments were conducted on established
forages in growers fields over 6 locations. Spray dates varied according to the
appropriate crop stage at each location.
Table 3.
Treatments for established alfalfa and birdsfoot trefoil trial.
|
Herbicide |
Rate/acre |
Water Volume/acre |
|
2,4D amine (600 g/L) |
.71 L |
90 L |
|
Basagran/Assist |
.91 L/.41 L |
80 L |
|
Embutox 625 |
.71 L |
90 L |
|
MCPB+MCPA |
1.11 L |
90 L |
|
Odyssey |
.012 kg |
45 L |
|
Pardner |
.405 L |
70 L |
|
Pardner+MCPA na+ |
.405 L/.36 L |
90 L |
|
Pursuit/Agsurf |
.085 L/.25 L per 100L of spray solution |
180 L |
|
Sencor 75 DF |
.111 L |
70 L |
|
Sundance/Merge |
.011 kg/.2L |
45 L |
*ALF alfalfa, BFT
birdsfoot trefoil
Note:
Water volumes
were the maximum recommended for each herbicide.

Crop stands were assessed for herbicide tolerance approximately four, seven and 37 DAT
and seed yield was measured for alfalfa.
Results and Discussion:
The established herbicide trial included crop tolerance as well as seed yield.
Established forage species often recovered from herbicide damage by 37 days after
treatment that their seedling counter parts did not. In some cases, presence of visible
crop damage did not translate into reduced seed yield. Alfalfa was less tolerant to 2,4 D
amine than Odyssey, however, seed yield for alfalfa sprayed with 2,4 D amine was no
different than that for alfalfa sprayed with Odyssey.
There was minor crop injury to established alfalfa when sprayed with 2,4 D amine, MCPB
+ MCPA and Pardner + MCPA, which are known to control top growth of Canada thistle and
dandelion (2,4 D amine only) (Figure 1 and 2). Birdsfoot trefoil tolerated Embutox and
MCPB + MCPA well, thus these products may provide the Canada thistle control trefoil
producers are looking for (Figure 3). Unfortunately, significant crop injury occurred to
alfalfa and birdsfoot trefoil when sprayed with Sundance, which means the search for good
quackgrass control in these crops continues.
Several herbicides of known efficacy on weeds were identified as having potential to
control weeds in legume seed crops. Some of these herbicides may provide the answer to the
weeds of biggest concern to forage seed producers. The herbicide screening trial was
designed in such a way as to act as a sieve with fairly large holes. A large number of
herbicides (23 on seedling crops) were screened over several forage species and cultivars.
Once herbicides are identified as having no adverse effect on crop tolerance and answer
specific weed problems in various forage seed crops will be entered into trials geared
toward minor use registration.

In 2001, minor use trials will be conducted in cooperation with forage seed
associations in Saskatchewan, Alberta and British Columbia.
Acknowledgements:
This research was initiated through the financial support of the Agri-Food Research and
Development Initiative (ARDI), Covering New Ground (CNG), Manitoba Rural Adaptation
Council (MRAC), and the Manitoba Forage Seed Association (MFSA).
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