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Background and Objectives:
The objective of the original proposal was two-fold:
- To determine the effect of
fertilizer timing, rate and formulation on grain protein in spring and winter wheat.
- To investigate palatability and feed value differences among a number of different
perennial forage species.
Price premiums are currently available for high grain protein content in spring and
winter wheat. However, producers want to achieve high protein without sacrificing yield
and ensuring there is an economic benefit. In recent years, protein premiums have been
significant and important since commodity base prices remain low. Questions are being
asked by producers whether split applications of nitrogen can translate into higher grain
protein. Research has shown that late-season nitrogen applications (after tilling to after
anthesis) have a greater impact on grain protein than grain yield. Much of this research
has focused on foliar nitrogen applications and in spring wheat. Therefore, this project
was designed to investigate whether mid-season applications of ammonium nitrate (34-0-0)
could impact the grain protein of spring or winter wheat.
Procedure and Project Activities:
Replicated small plots were established in 1999 and 2000. Plots were approximately 5 m
X 5 m in size and there were four replicates. The two trials (winter and spring wheat)
were established at two sites. Two varieties of winter wheat (CDC Clair and CDC Kestrel)
were seeded at Rosebank, MB (sandy loam site) and Crystal City (clay loam site). Three
fertilizer treatments were applied to the winter wheat: 1) 100 lbs N at green-up in spring
2) 100 lbs N at green-up plus 25 lbs N at flowering and 3) 125 lbs N at green-up.
Measurements included: yield and grain protein determination (elevator and lab analysis).
Three varieties of spring wheat were seeded in 1999 (AC Majestic, AC Barrie and AC
Cora). Three fertilizer treatments applied were: 1) 80 lbs N banded at seeding 2) 80 lbs N
banded at seeding plus 20 lbs N at flowering and 3) 100 lbs N banded at seeding.
Measurements included: yield and grain protein determination (lab analysis only).
In 2000, the winter wheat did not establish at the Crystal City site (winter killed)
and had to be abandoned. A fertilizing error at Rosebank resulted in the spring wheat
trial to discontinue at Rosebank in 2000. A late season hail storm caused significant
damage at the Crystal City site so the spring wheat trial was not taken to yield.
Therefore, the only data obtained in 2000 was from the winter wheat trial at Rosebank.
Mid-season fertilizer treatments were broadcast applied (see photo 1). Recommended
agronomic practices (e.g. seeding rate, herbicides, fungicides, etc.) were used throughout
the project. Protein analysis was conducted by Norwest Labs and at a major grain handling
facility.

Photo 1. Mid-season application of ammonium nitrate to winter wheat.
Results and Discussion:
Spring Wheat 1999
Data tables for the spring wheat are illustrated in Appendix
A (Crystal City 1999) and Appendix B (Rosebank 1999). No statistical differences were
detected in the spring wheat data analysis. All three varieties responded similarly at
both locations. Yield and protein were unaffected by the different fertilizer rates and
timings. Therefore, in this trial, the spring wheat did not respond to the additional
nitrogen applied mid-season. Protein premiums begin to show larger returns per bushel when
grain protein tests higher than 13.0 % (up to 15.5). Generally, all three varieties at
both locations tested higher in protein than 13.0 %. Both locations tested low in soil
nitrogen prior to seeding and very little nitrogen remained in the soil post-harvest. This
indicates that even the lowest rate of fertilizer was adequate at achieving high yield
while maintaining protein content. Perhaps a larger range of treatments would have
resulted in more critical levels being determined.
Winter Wheat 1999
No significant differences were detected in wheat yield at Rosebank (Appendix C). Both varieties yielded similarly under the three different
fertilizer regimes. At Crystal City, the 100 lbs N at green-up plus 25 lbs N at flowering
yielded highest for both CDC Clair and CDC Kestrel. This treatment yielded significantly
higher than the other two (which were not different from each other). In 1999, therefore,
the winter wheat crop at Crystal City responded to the addition of mid-season nitrogen
application in the form of yield.
The additional nitrogen applied mid-season, however, appeared to increase grain protein
more consistently at both sites (Appendix D). At Rosebank both varieties of winter wheat
and at Crystal City CDC Clair accumulated the greatest grain protein treatment #2 (100 lbs
N at green-up plus 25 lbs N at flowering/anthesis). All three of these proteins were
significantly greater than the other two treatments (which were not significantly
different from each other). At Crystal City, no significant difference in grain protein
(lab or elevator analysis) was detected in any treatments applied to CDC Kestrel variety.
The winter wheat crop responded with both a yield and protein increase to the mid-season
fertilizer application.
Winter Wheat 2000
The yield of CDC Kestrel winter wheat was significantly lower
in treatment # 3 (125 lbs N at green-up) than the other two treatments (Appendix E). No
scientific explanation can be given for this occurring. The other two treatments in the
CDC Kestrel and all three treatments applied to CDC Clair did not result in statistical
yield differences. Although treatment # 2 appeared to yield the highest when absolute
values are considered. No significant differences in grain protein were detected among the
three treatments on either winter wheat variety. The response to mid-season fertilizer
application as seen in 1999 did not occur. Timely rains in 1999 would have resulted in the
broadcast application of 34-0-0 being carried into the soil and root zone encouraging root
uptake. Extremely dry conditions in July and August in 2000 after the mid-season
application of fertilizer could explain the lack of response.
Elevator vs. Lab Protein Analysis
In 1999, there was a relatively large
discrepancy between the elevator protein test result compared to the lab analysis result
(Appendix F). On average (including both sites, varieties and treatments) the difference
ranged from 1.7 to 2.1% protein with the lab analysis being consistently higher. Protein
premiums for winter wheat begin at 11.5%. The discrepancy between the elevator and lab
protein analysis was large enough in some treatments to impact achieving vs. not achieving
the protein premium level of 11.5% (the elevator analysis would be lower, but the lab
analysis would be higher). The elevator protein tester had been calibrated for winter
wheat, but continued to test consistently lower. In 2000, the lab analysis again
consistently tested higher than the elevator method, but the difference was not as large
(0.2 to 0.5%) as in 1999. After discussion with the elevator company in 1999, it was
suggested that a larger harvested grain sample (manufacturer recommends minimum of 250 g)
be used for protein determination via the elevator method. This was taken into account in
2000 and as a result the difference between the two test methods was not as large.
The spring wheat varieties did not respond to the different rates or timing of
fertilizer in this trial. Perhaps a larger range of rates would have produced different
results. It appears that the winter wheat varieties did respond with yield and protein
increases when additional nitrogen was applied mid-season (at anthesis). Previous research
by others has shown that mid-season nitrogen uptake must be through the root system
(whether foliar applied or broadcast). Therefore, a response to mid-season nitrogen
application is dependent on rainfall occurring after application to increase uptake.
Acknowledgements:
Funding for this project was obtained from the Agri-Food Research and Development
Initiative (ARDI). Technical assistance from Manitoba Agriculture and Food is gratefully
acknowledged. In-kind support from Westco, Agricore and Cargill Grain is also gratefully
acknowledged.
Appendices:
Appendix A -
Spring
Wheat Results at Crystal City in 1999
Table 1. Yield and Protein of AC Majestic
Spring Wheat at
Crystal City in 1999.
|
Fertilizer Treatment (lbs N per
acre) |
Yield (bushels/acre) |
Protein (%) |
|
80
spring applied |
45.4 a |
15.5 a |
|
80
spring applied + 20 at flowering |
42.9 a |
15.8 a |
|
100 spring applied |
41.1 a |
15.6 a |
Table 2. Yield and protein of AC Barrie spring wheat
at Crystal City in 1999.
|
Fertilizer
Treatment (lbs N per acre) |
Yield
(bushels/acre) |
Protein (%) |
|
80
spring applied |
39.4 a |
15.6 a |
|
80
spring applied + 20 at flowering |
37.6 a |
15.9 a |
|
100 spring applied |
36.6 a |
15.8 a |
Table 3. Yield and protein of AC Cora spring wheat at
Crystal City in 1999.
|
Fertilizer
Treatment (lbs N per acre) |
Yield
(bushels/acre) |
Protein (%) |
|
80
spring applied |
45.6 a |
16.3 a |
|
80
spring applied + 20 at flowering |
46.6 a |
16.1 a |
|
100 spring applied |
48.6 a |
16.3 a |
Appendix B. Spring Wheat Results at
Rosebank in 1999
Table 1. Yield and protein of AC Majestic spring wheat at
Rosebank in 1999.
|
Fertilizer
Treatment (lbs N per acre) |
Yield
(bushels/acre) |
Protein (%) |
|
80
spring applied |
38.0 a |
14.1 a |
|
80
spring applied + 20 at flowering |
39.2 a |
13.9 a |
|
100 spring applied |
41.0 a |
14.4 a |
Table 2. Yield and protein of AC Barrie spring wheat
at Rosebank in 1999.
|
Fertilizer
Treatment (lbs N per acre) |
Yield
(bushels/acre) |
Protein (%) |
|
80
spring applied |
39.0 a |
12.9 a |
|
80
spring applied + 20 at flowering |
42.7 a |
13.7 a |
|
100 spring applied |
39.0 a |
14.1 a |
Table 3. Yield and protein of AC Cora spring wheat at
Rosebank in 1999.
|
Fertilizer
Treatment (lbs N per acre) |
Yield
(bushels/acre) |
Protein (%) |
|
80
spring applied |
46.3 a |
14.2 a |
|
80
spring applied + 20 at flowering |
42.9 a |
14.3 a |
|
100 spring applied |
46.4 a |
15.2 a |
Appendix C. Effect of Fertilizer Rate and Timing on Winter Wheat Yield in 1999
Appendix D. Effect of Fertilizer
Rate and Timing on Winter Wheat Protein in 1999
Appendix E. Effect of Fertilizer
Rate and Timing on Winter Wheat Yield and Protein in 2000
Appendix F. Winter Wheat Protein
Comparisons (Elevator vs. Lab Analysis)
Table 1. Elevator and Lab Protein Analysis of Winter Wheat
at Rosebank in 1999.
|
Fertilizer Treatment
(lbs N per acre) |
CDC Clair |
CDC Kestrel |
|
Elevator Protein (%) |
Lab Protein (%) |
Elevator Protein (%) |
Lab Protein (%) |
|
100 spring applied |
7.6 a |
9.8 a |
7.6 a |
9.6 a |
|
100 spring + 25 at flowering |
9.5 b |
11.7 b |
9.1 b |
11.3 b |
|
125 spring applied |
8.3 a |
10.4 a |
7.8 a |
9.6 a |
Table 2. Elevator and Lab Protein Analysis of Winter
Wheat at Crystal City in 1999.
|
Fertilizer Treatment
(lbs N per acre) |
CDC Clair |
CDC Kestrel |
|
Elevator Protein
(%) |
Lab Protein (%) |
Elevator Protein
(%) |
Lab Protein (%) |
|
100 spring applied |
10.7 a |
12.3 a |
10.2 a |
11.9 a |
|
100 spring + 25 at flowering |
12.4 b |
14.4 b |
10.7 a |
12.3 a |
|
125 spring applied |
10.9 a |
12.5 a |
10.9 a |
12.5 a |
Table 3. Elevator and Lab Protein Analysis of Winter
Wheat at Rosebank in 2000.
|
Fertilizer
Treatment
(lbs N per acre) |
CDC Clair |
CDC Kestrel |
|
Elevator Protein
(%) |
Lab Protein (%) |
Elevator Protein
(%) |
Lab Protein (%) |
|
100 spring applied |
10.1 a |
10.5 a |
9.9 a |
10.4 a |
|
100 spring + 25 at flowering |
11.5 a |
11.3 a |
10.3 a |
10.6 a |
|
125 spring applied |
10.3 a |
10.6 a |
9.7 a |
10.4 a |
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